When do you need to fix smears for cytological examination?
Smears must be fixed immediately before they dry. A 95% solution of ethyl alcohol or spray fixative is used to fix the slide. The slides should be labeled in pencil with the patient’s name AND date of birth and a properly completed Pap smear requisition should accompany the slide.
How do you fix cytology smear?
2 The commonly used methods are air-dried and wet-fixed smears. Air- dried smears have many advantages over wet-fixed smears during routine cytology. They may be post- fixed after rehydration in saline with a variety of fixatives, such as ethanol/acetic acid, 95% ethanol or alcoholic formalin.
What is the best time to collect cytological materials?
Cytology specimens received in the laboratory after 2:30 p.m. on weekdays and on weekends or holidays will be evaluated by laboratory staff to see that they are properly preserved to prevent cellular degeneration.
How are cytology specimens processed?
Processing cytology samples involves reception of the specimen and request form, preparation of slides for microscopic examination, staining, screening and reporting the slides. All these processes are subject to quality control and quality assurance measures.
How do you perform a cytology test?
A urine cytology test requires a urine sample, which you provide by urinating into a sterile container. In some cases, a urine sample is collected using a thin, hollow tube (catheter) that’s inserted into your urethra and moved up to your bladder.
How do you perform a cytology?
Another cytology technique is to gently scrape or brush some cells from the organ or tissue being tested. The best-known cytology test that samples cells this way is the Pap test. A small spatula and/or brush is used to remove cells from the cervix (the lower part of the uterus or womb) for a Pap test.
What is wet fixative?
1 For wet fixation numerous fixatives are used in exfoliative cytology of which 95% ethyl alcohol is most commonly used one. 2 The commonly practiced method is to dip the freshly spread FNAC smear immediately in 95% ethanol. 3 Other methods of smear fixation includes spray fixatives.
Why do we fix cytological specimen?
Immediate fixation of cytology specimens is critical to the preservation of the cellular components. It is important that no air-drying occurs prior to fixation. If a smear is already air- dried it should NOT be put in alcohol fixative.
What is the primary purpose of cytology evaluation?
Cytology is often used to diagnose growths or masses (tumors) found on the surface of the body, but can also be used to assess bodily fluids, internal organs (e.g., liver, lung, lymph nodes, kidney), and abnormal fluids that may accumulate, especially in the chest and abdomen.
What is the difference between cytology and biopsy?
However, the emphasis in cytological examination is on cellular changes. In biopsy, we can study tissue patterns and inter-relationships as well as cellular changes.
What can you do to decrease blood contamination of your cytology slides?
Rapidly drying the slides (hairdrier or by ‘waving’ in the air) after preparation, reduces cell crenation & slow -drying artefact, resulting in superior cell preservation. 10. Simply spraying material onto the slide often produces thick cell clumps, which will impede individual cell cytological evaluation. 11.
What is the preferred fixative for most cytology specimens?
Fixative: 95% ethyl alcohol is recommended.